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Microsoft Word - Yoshida10-03proof.doc
eases, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan TEL: +81-6-6879-3833, Fax: +81-6-6879-3839, E-mail address: hiroinosaka@hotmail.com
ABSTRACT
4-Hydroxy-2-nonenal (4-HNE), [...] experiments, the cells were preincubated with various re- agents for 30 min and exposed to 4-HNE for 6 hr. Cell viability was determined us- ing a Cell Counting Kit-8 (Dojindo Labo- ratories, Kumamoto, Japan) [...] exposed to 50 μM 4-HNE. Graphic results are expressed as a percentage of control value, mean ± SD (n=6). WST-8 assay and LDH assay of HUVECs treated with or without (C) 10 U/ml SOD or (D) 1000 U/ml catalase …