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two-state model, while α-lactalbumin unfolding starts with a two- state mechanism (when [GdnHCl]≥1.6M) followed by a three-state pathway.
Keywords: Protein denaturation, spectrophotometry, two-state d [...] purchased from Merck. The solutions were in distilled water. KCl (0.1 M) and phosphate buffer (20 mM, pH=6.8) for α- lactalbumin solution and formate buffer (30 mM, pH=3.55) for ribonuclease A was used. Protein [...] protein unfolding is equal to:
)(,,, jjijjijiijiji DNDNAAA +−+=−=Δ εεε (5)
jijiji DA )( ,, εε −=Δ (6)
For another wavelength, ii, with similar notations,
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jiijiijii DA )( ,, εε −=Δ (7)
Therefore, there …