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rt; b) 4eq.PyClocK,4eq Fmoc-NH-aa-COOH,4±8eqDIPEAin DMF, 2h, rt; for non-naturalaa:3±4eq.PyClocK,3eq.Fmoc-NH-aa-COOH, 4±6eq.DIPEAin DMF, 2h, rt; c) 10mol%1stgen.Grubbscatalystin DCE,3x 1h, rt; d) 4eq.Ac2O [...] B:Sequenceof stapled peptides1±5derivedfrom Nrf2. Derivative1 (i,i+4 stapling); derivative2 (i,i+4 stapling);derivative3 (i,i+3 stapling); derivative4 (i,i+7 stapling).Colorcodingaccordingto Fig1.
https://doi.org/10 [...] potentialinteractionwith cellularDNA, weincubatedpeptidesFITC-4 andFITC-5with fixed,permeabilizedHeLacells.Asshownin Fig4,peptideFITC-4wasselec- tivelyenrichedin theDNA-rich nucleus.In contrast,only …